The presence of starch is confirmed through the iodine test by observing a distinct color change when iodine solution is introduced. This change, typically to a dark blue or black hue, serves as the indicator of a successful interaction between iodine and starch molecules. The material known to elicit this specific, expected color change is a crucial element in validating the entire testing procedure.
Employing a substance with a known starch content is vital to ensure the reagents are functioning correctly and the test is being conducted properly. This practice allows for the verification that a negative result is genuinely due to the absence of starch, rather than a flaw in the methodology. Historically, this quality control measure has been integral to food science, biochemistry, and educational laboratories, ensuring accurate assessments of starch presence in diverse samples.